Effects of natural antimicrobial compounds propolis and copaiba on periodontal ligament fibroblasts, molecular docking, and in vivo study in Galleria mellonella.

Root canal treatment addresses infectious processes that require control. Occasionally, the radicular pulp is vital and inflamed, presenting a superficial infection. To preserve pulpal remnants, conservative procedures have gained favor, employing anti-inflammatory medications. This study investigated the effects of propolis (PRO), and copaiba oil-resin (COR) associated with hydrocortisone (H) and compared their impact to that of Otosporin® concerning cytotoxic and genotoxic activity, cytokine detection, and toxicity in the Galleria mellonella model. Human periodontal ligament fibroblasts (PDLFs) were exposed to drug concentrations and evaluated by the MTT assay. Associations were tested from concentrations that did not compromise cell density. Genotoxicity was evaluated through micronucleus counting, while cytokines IL-6 and TGF-ß1 were detected in the cell supernatant using ELISA. Molecular docking simulations were conducted, considering the major compounds identified in PRO, COR, and H. Increasing concentrations of PRO and COR were assessed for acute toxicity in Galleria mellonella model. Cellular assays were analyzed using one-way ANOVA followed by Tukey tests, while larval survivals were evaluated using the Log-rank (Mantel-Cox) test (α = 0.05). PRO and COR promoted PDLFs proliferation, even in conjunction with H. No changes in cell metabolism were observed concerning cytokine levels. The tested materials induce the release of AT1R, proliferating the PDFLs through interactions. PRO and COR had low toxicity in larvae, suggesting safety at tested levels. These findings endorse the potential of PRO and COR in endodontics and present promising applications across medical domains, such as preventive strategies in inflammation, shedding light on their potential development into commercially available drugs.

Linalool production from the leaves of Bursera aloexylon and its antimicrobial activity.

The oil of the leaves of Bursera aloexylon was found to contain a high linalool level (96.7 %). The antimicrobial activity tests indicated that the oil was effective against Rhodococcus equi (0.60 mg/ml) and Staphylococcus epidermides (0.15 mg/ml).

Evaluation of paraffins biodegradation and biosurfactant production by Bacillus subtilis in the presence of crude oil

Os experimentos com Bacillus subtilis para avaliacão da tensão superficial foram realizados com meio de cultivo contendo como nutrientes básicos 0,4 por cento de ions nitrato e 4 por cento de glicose, na presenca de petróleo. A producão de surfactina foi observada pela reducão da tensão superficial do meio de cultura fermentado. Surfactina foi isolada a partir do meio de cultura fermentado por B. subtilis, por precipitacão ácida seguida de extracão com clorofórmio-metanol. A avaliacão da composicão dos alcanos lineares (parafinas) foi realizada por cromatografia gasosa. Observamos uma significativa reducão da tensão superficial do meio de cultura indicando que a producão de biosurfactante não foi inibida pela presenca de parafina, e que as parafinas leves podem ter sido consumidas.